Ngwaahịa

Nkọwa ngwaahịa:

Ebumnuche Innovita® 2019-nCoV IgM/IgG bụ maka nyocha na nleba anya ọrịa ọrịa nke coronavirus ọhụrụ (2019-nCoV) kpatara.A na-achọpụta ORF1ab na N gene nke 2019-nCoV nke ọma site na akpịrị akpịrị na ihe nlele mmiri alveolar lavage anakọtara site na ndị a na-enyo enyo na oyi baa, ndị ọrịa nwere ọrịa a na-enyo enyo, ndị ọzọ kwesịrị ịchọpụta.
Nsonaazụ ule nke ngwa a bụ naanị maka ntụle aka ụlọọgwụ.A na-atụ aro ka ịme nyocha zuru oke nke ọnọdụ ahụ dabere na ngosipụta ụlọ ọgwụ nke onye ọrịa na nyocha ụlọ nyocha ndị ọzọ.

Ụkpụrụ:

Ngwa a na-eji mmeghachi omume transcription polymerase chain otu-nzọụkwụ (RT-PCR) teknụzụ nchọpụta iji lekwasịrị anya n'akwụkwọ akụkọ coronavirus (2019-nCoV) ORF1ab gene, N gene, na usoro nrụtụ aka n'ime mmadụ.Emebere ngwa ngwa na nyocha taqman na mpaghara echekwara.

Ihe mejupụtara:

Cheta na: 1. Dị iche iche batches nke reagents ekwesịghị agwakọta.
2. njikwa dị mma na njikwa dị mma adịghị mkpa ka ewepụtara ya

Usoro nnwale:

1. Nucleic acid mmịpụta:
Ngwa mmịpụta RNA azụmahịa dị, a na-atụ aro mmịpụta bead magnet na mmịpụta kọlụm maka ngwa a.
2. Kwadebe ngwakọta mmeghachi omume:

● Wepụ mmeghachi omume 2019-nCoV Mix A/B ma debe n'ime ụlọ okpomọkụ ruo mgbe oyi na-ekpo ọkụ;
● Were akụkụ ndị kwekọrọ na ya (Reaction Mix A 16.5μL / T, Reaction Mix B 3.5μL / T) na mix, na mgbe ahụ, aliquot ọ bụla PCR mmeghachi omume na 20μL / tube;
● Tinye 5μL nke template RNA ma ọ bụ njikwa na-adịghị mma ma ọ bụ njikwa dị mma, wee kpuchie okpu tube;
● Tinye tube mmeghachi omume n'ime ngwa PCR fluorescence, ma tọọ njikwa na-adịghị mma / nke dị mma na ihe nlele maka mmeghachi omume RT-PCR dị ka ntuziaka ọrụ ngwá ọrụ si dị.
● Dekọọ usoro ntinye ihe nlele

3.RT-PCR ụkpụrụ:

Ntọala akwadoro: